applications

Stanford_10_mouse

Sharon S. Hori, Sheen-Woo Lee, Sanjiv Sam Gambhir (Canary Center at Stanford, Stanford University School of Medicine)

The versatility, ease of use and quantitative capabilities of SI Imaging systems make these instruments well suited for a wide range of applications.* Let a Kino, Ami or Lago system address your imaging needs.

Small Animal Imaging In Vivo

newmousefluor-xray_optimized-xraySmall animal optical imaging is one of the most challenging applications for a laboratory system. Optical photons are strongly attenuated in deep tissue, making it necessary for a system to achieve the highest sensitivity possible. Furthermore, it is typically the intensity of the light source that is of interest, so it is necessary to provide a stable and well calibrated optical system. The Kino, Ami and Lago systems, with both luminescent and fluorescent as well its optional X-ray capability, provides an easy way to image plates, mice, rats, insects or fish and make quantitative measurements of optical intensity on co-registered optical or X-ray images. This capability addresses the needs for research and development in oncology, drug development, toxicology, infectious diseases, and a host of other applications.

The top image displays the detection of fluorescent signals within mice. Five mice were subcutaneously injected with LiCor IRDye 800 CW Carboxylate and imaged for 20 seconds. Fluorescent signals are shown overlaid onto a photographic image. [745 nm excitation, 845 nm emission, f/2].

The bottom portion of the image shows the X-ray image of mice with fluorescent signal overlay. [40 kV, 20 sec X-ray exposure].

In Vitro
96wellAll

Optical reporters, both luminescent and fluorescent, play a huge role in genetic, proteomic and biological research. The high sensitivity and absolute calibration of the Kino, Ami and Lago systems make quantitative well plate measurements fast and easy. The system can also be used for colony counting and cell viability measurements. The versatility of the bioluminescent and fluorescent imaging modes conveniently allows the use of almost any reporter or dye, including green, red, and infra-red markers.

Serially-diluted fluorescent dye. A 60 sec image of a 96-well plate with a 1:2 dilution series of LiCor IRdye 800CW carboxylate dye is overlaid overlaid onto a photographic image. [ 745 nm excitation, 845 nm emission, f/1.2]

Plants

Fluorescent imaging of plants and seedlings has become a valuable tool to investigate multiple aspects of plant biology and genetics. The Kino, Ami and Lago systems permit rapid evaluation and localization of fluorescent indicators to help in the understanding plants-a-b (1)
of plant gene expression, growth and development, circadian rhythm, chemical resistance, and stress response.

Fluorescent emissions from desert flora. A flowering plant and cactus were imaged for 20 sec with 465 nm excitation. Pseudocolor fluorescent tomato-filter3 (1)

signals were overlaid onto a photographic image. Panel A was imaged at 550 nm emission; panel B was imaged at 735 nm emission. 10s images of tomato plant in group mode. Psuedocolor luminescence imaging overlaid onto photographic image.

Food Safety

In support of food safety program with the University of Arizona Dept of Veterinary Science and Microbiology.

E_coli_in_PB (1)

Bioluminescence E. coli in peanut butter, 60s image. Psuedocolor luminescence imaging overlaid onto photographic image. University of Arizona Dept of Veterinary Science and Microbiology.

Cerenkov Luminescence Imaging

The SI Imaging line of imaging devices enables capturing of the visible photons emitted by Cerenkov radiation.

cerenkov-imaging (3)

Image provided by and credited to Van Andel Research Institute

General Imaging

Fluorescence emission from genetically modified zebrafish overlaid on X-ray imaged utilizing the Ami.

gloFish1_4w (1)

* SI Imaging systems can be used for a wide variety of applications, including in vivo and in vitro molecular imaging, purchasers should be aware that some methods and applications for molecular imaging may be subject to various patent rights